Optimization of polymerase chain reaction for monitoring of Borrelia burgdorferi infection by ixodid ticks

Determination of the infection rate ixodid ticks with tick-borne borreliosis pathogens and determination belonging to pathogenic genotype by PCR is an important component for monitoring, risk assessment control epizootic situation Lyme in different territories. The results testing optimization internal laboratory protocol classical polymerase chain reaction identification disease are presented. Eight samples extracted DNA from collected vegetation forest park tract "Golendernya", Bila Tserkva, Kyiv region, were examined PCR. Samples formed pools ten tick specimens: seven - genus I. ricinus one pool D. reticulatus. For detection borrelia DNA, primer sets used detect Borrelia burgdorferi sensu lato complex; borrelia: stricto, garinii afzelii. nucleic acid extraction was modified using commercial IndiSpin Pathogen Kit. Optimization amplification temperature conditions carried out annealing gradient method each pair. Based on study, protocols specific oligonucleotide primers developed. It found that reticulatus there infected specimens complex afzelii genus, also identified stricto reticulatus, not detected. developed will be further study pathogens: lato, Key words: borreliosis, Ixodes ticks, reaction, afzelii, garinii.